Isolation and Culture of Mouse Primary Pancreatic Acinar Cells
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چکیده
منابع مشابه
Isolation and culture of mouse primary pancreatic acinar cells.
This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it possible to maintain them in culture for more than one week. More than 20 x 10(6) acinar cells can be obtained from a single murine pancreas. This protocol offers the possibility to independently process as many as 10 pancreases in parallel. Because it preserves acinar architecture, this model is wel...
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INTRODUCTION The methodology of acinar cell culture has become of primary importance in the research of pancreatic physiology and pharmacology. AIM To develop a method for primary culture of porcine pancreatic acinar cells. METHODOLOGY Dispersed pancreatic acinar cells were made by RPMI-1640 medium containing collagenase III. After purification, the isolated acinar cells were cultured in RP...
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We have investigated control mechanisms involved in the propagation of agonist-induced Ca2+ waves in isolated mouse pancreatic acinar cells. Using a confocal laser-scanning microscope, we were able to show that maximal stimulation of cells with acetylcholine (ACh, 500 nM) or bombesin (1 nM) caused an initial Ca2+ release of comparable amounts with both agonists at the luminal cell pole. Subsequ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2013
ISSN: 1940-087X
DOI: 10.3791/50514